- "The tacky bow, the beady eyes—you're just hideous!"
- —Muffy Crosswire rudely describing Prunella.
Prunella lives with her mother, Wanda Deegan, and her much older sister, Rubella Deegan. Her mother is single after breaking up with Prunella's father, Nigel Ratburn, a rat. The inter-species union makes Prunella and her sister rare halfbreeds.
Prunella and her family seem to practice the Rat God religion, where they worship a rat deity. Prunella was once considered the sole prophet of the religion.
Prunella was born to Wanda Deegan and Nigel Ratburn in 2004 or 2005, after the couple already had Prunella's sister, Rubella Deegan. Her mother is a poodle and her father is a rat, making her a halfbreed. Sometime after Prunella's birth and before 2014, her father and moved informally seperated, because it was dangerous to be an inter-species couple before it was legal. Prunella did not know who her father was until 2014.
Prunella is a scrawny rat-like poodle. She is in fourth grade, unlike many of the other characters who are in third grade. She has puffy hair and she wears a bow, and a button-down dress with a collar. Unlike the other characters, Prunella's eyes are small and beady. This was addressed in the issue Do You See What I See?
- Prunella the Sewer Rat
- Do You See What I See?
- Voodoo Queen
- Fern Finally Cracks
- The Fat Girl in the Mirror
- Killer & Me
- The Exorcism
- The Unthinkable
- The Church of Prunella, Part 1
- The Church of Prunella, Part 2
- The Church of Prunella, Part 3
- Because of Killer
- Marina Sees the Light
- A Special Prom
- A Special Prom: Redux